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enrichKEGG_pip.r KEGG 富集分析
$Rscript $scriptdir/enrichKEGG_pip.r -h usage: /work/my_stad_immu/scripts/enrichKEGG_pip.r [-h] -g gene.list [-d ann.db] [-t idtype] [-s show.type] [-O organism] [-p pvalueCutoff] [-q qvalueCutoff] [-c showCategory] [-n prefix] [-o outdir] [-H height] [-W width] KEGG enrich analysis : https://www.億速云.com/article/1503 optional arguments: -h, --help show this help message and exit -g gene.list, --gene.list gene.list diff expressed gene list file, required -d ann.db, --ann.db ann.db org.Hs.eg.db or org.Mm.eg.db ,for more info visit: https://www.億速云.com/article/1244 [optional, default: org.Hs.eg.db ] -t idtype, --idtype idtype deg gene id type: ENSEMBL SYMBOL ENTREZID GENENAME [optional, default: SYMBOL ] -s show.type, --show.type show.type set example ID type name [optional, default: ENTREZID ] -O organism, --organism organism organism ,for more info visit: https://www.億速云.com/article/787 [optional, default: hsa ] -p pvalueCutoff, --pvalueCutoff pvalueCutoff pvalue cutoff on enrichment tests to report, [optional, default: 0.05 ] -q qvalueCutoff, --qvalueCutoff qvalueCutoff qvalue cutoff on enrichment tests to report as significant. Tests must pass i) pvalueCutoff on unadjusted pvalues, ii) pvalueCutoff on adjusted pvalues and iii) qvalueCutoff on qvalues to be reported., [optional, default: 0.2 ] -c showCategory, --showCategory showCategory how many KEGG Category to show, [optional, default: 10 ] -n prefix, --prefix prefix the output file prefix [optional, default: KEGG ] -o outdir, --outdir outdir output file directory [default cwd] -H height, --height height the height of pic inches [default 5] -W width, --width width the width of pic inches [default 7]
-g 輸入基因列表文件: 腳本會(huì)讀取第一列基因ID作為基因集:
-d 物種注釋數(shù)據(jù)庫: 人的:org.Hs.eg.db
-t 指定輸入基因列表的ID類型
Rscript $scriptdir/enrichKEGG_pip.r --gene.list $workdir/04.deg/S1_vs_S2.DEG.final.tsv \ -o KEGG -n S1_vs_S2 --pvalueCutoff 0.01 --ann.db org.Hs.eg.db --organism hsa \ --idtype SYMBOL
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