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這篇文章主要介紹“R語(yǔ)言的ppi_network.r怎么用”,在日常操作中,相信很多人在R語(yǔ)言的ppi_network.r怎么用問(wèn)題上存在疑惑,小編查閱了各式資料,整理出簡(jiǎn)單好用的操作方法,希望對(duì)大家解答”R語(yǔ)言的ppi_network.r怎么用”的疑惑有所幫助!接下來(lái),請(qǐng)跟著小編一起來(lái)學(xué)習(xí)吧!
ppi_network.r 蛋白互作網(wǎng)絡(luò)分析
$ Rscript $scriptdir/ppi_network.r -h usage: /work/my_stad_immu/scripts/ppi_network.r [-h] -g gene.list [-s ann.db] [-t score_threshold] [-v version] [-p prefix] [-o outdir] [-H height] [-W width] GO enrich analysis optional arguments: -h, --help show this help message and exit -g gene.list, --gene.list gene.list diff expressed gene list file, required -s ann.db, --species ann.db NCBI taxonomy identifiers of the organism (e.g. 9606 for Human, 10090 for mouse). default 9606 -t score_threshold, --score_threshold score_threshold a threshold for the combined scores of the interactions, such that any interaction below that threshold is not loaded in the network (by default the score threshold is set to 700) -v version, --version version define the STRING version to be used [optional, default: 11 ] -p prefix, --prefix prefix the output file prefix [optional, default: PPI ] -o outdir, --outdir outdir output file directory [default cwd] -H height, --height height the height of pic inches [default 10] -W width, --width width the width of pic inches [default 10]
-g 指定基因列表文件為SYMBOL ID :讀取文件的第一列為ID列表:STRING數(shù)據(jù)庫(kù)獲取網(wǎng)絡(luò);
Rscript $scriptdir/ppi_network.r -g ../04.deg/S1_vs_S2.DEG.final.tsv -p S1_vs_S2
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